Designs and audits PCR and RT-qPCR primers with Primer3, explicit thermodynamic conditions, reference-based off-target amplification searches, and traceable sequence coordinates. Use for designing primer pairs, checking existing primers, exon-junction or isoform-specific assays, variant masking, cloning tails, multiplex compatibility, and interpreting Primer-BLAST results.
Install
npx skills add K-Dense-AI/scientific-agent-skills --skill primer-designnpx skills add K-Dense-AI/scientific-agent-skills --skill primer-design -a claude-code -gnpx skills add K-Dense-AI/scientific-agent-skills --skill primer-design -a codex -ggemini skills install https://github.com/K-Dense-AI/scientific-agent-skills.git --path skills/primer-designgh skill install K-Dense-AI/scientific-agent-skills primer-designgit clone --depth 1 https://github.com/K-Dense-AI/scientific-agent-skills /tmp/K-Dense-AI__scientific-agent-skills
mkdir -p ~/.claude/skills ~/.agents/skills
cp -R /tmp/K-Dense-AI__scientific-agent-skills/skills/primer-design ~/.claude/skills/primer-design # Claude Code
cp -R /tmp/K-Dense-AI__scientific-agent-skills/skills/primer-design ~/.agents/skills/primer-design # Codex, Cursor, Gemini CLI, Copilot, OpenCode